7X53
cytochrome P450 monooxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 288.15 |
| Detector technology | PIXEL |
| Collection date | 2022-01-19 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.831, 55.630, 88.023 |
| Unit cell angles | 90.00, 102.94, 90.00 |
Refinement procedure
| Resolution | 23.510 - 3.350 |
| R-factor | 0.2724 |
| Rwork | 0.270 |
| R-free | 0.32060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.510 | 3.620 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 10361 | 2188 |
| <I/σ(I)> | 2.94 | |
| Completeness [%] | 94.5 | |
| Redundancy | 4.92 | |
| CC(1/2) | 0.989 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.5, 25% Polyethylene glycol 3350, 0.1 M TCEP hydrochloride |
