7X4Z
Crystal structure of Rhodostomin ARGDWP mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2019-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 67.709, 79.281, 92.011 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.800 - 1.480 |
| R-factor | 0.1652 |
| Rwork | 0.163 |
| R-free | 0.21392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rqg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.491 |
| Data reduction software | HKL-2000 (2.3.10) |
| Data scaling software | HKL-2000 (2.3.10) |
| Phasing software | HKL-3000 (2.3.10) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.070 | 0.714 |
| Number of reflections | 20726 | 2042 |
| <I/σ(I)> | 28.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 2M (NH4)2SO4 |
