7X4X
BTB domain of KEAP1 in complex with MEF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-01-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.58 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.322, 99.289, 143.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.390 - 2.960 |
R-factor | 0.2076 |
Rwork | 0.203 |
R-free | 0.26060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cxi |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.390 | 3.100 |
High resolution limit [Å] | 2.960 | 3.000 |
Number of reflections | 25315 | 2191 |
<I/σ(I)> | 8 | |
Completeness [%] | 98.1 | |
Redundancy | 5.1 | |
CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M lithium acetate, 20% PEG 3350 |