7X4Q
LpCdnE UTP Mg complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.584, 74.719, 80.343 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 27.230 - 1.950 |
| R-factor | 0.1627 |
| Rwork | 0.161 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7x4f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.075 | 0.513 |
| Rpim | 0.044 | 0.306 |
| Number of reflections | 56124 | 5577 |
| <I/σ(I)> | 17.5 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | UTP, MgCl2, PEG 4000, ammonium acetate, sodium citrate |
