7X4F

Native CD-NTase LpCdnE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-10
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 31
Unit cell lengths	60.507, 60.507, 100.588
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.970 - 2.460
R-factor	0.1928
Rwork	0.191
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7x4a
RMSD bond length	0.003
RMSD bond angle	0.483
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.077	0.629
Rpim	0.047	0.378
Number of reflections	14892	1507
<I/σ(I)>	16.5	2.1
Completeness [%]	99.0	100
Redundancy	3.7	3.8
CC(1/2)		0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	MgCl2, Tris, PEG 8000