7X4F
Native CD-NTase LpCdnE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-10 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.507, 60.507, 100.588 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.970 - 2.460 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7x4a |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.483 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.077 | 0.629 |
| Rpim | 0.047 | 0.378 |
| Number of reflections | 14892 | 1507 |
| <I/σ(I)> | 16.5 | 2.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | MgCl2, Tris, PEG 8000 |
