7X4C
Native CD-NTase EfCdnE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.322, 57.104, 64.845 |
| Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
| Resolution | 26.980 - 1.750 |
| R-factor | 0.1695 |
| Rwork | 0.168 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7x4a |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.653 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.033 | 0.075 |
| Rpim | 0.022 | 0.049 |
| Number of reflections | 29511 | 2981 |
| <I/σ(I)> | 35.1 | 15.1 |
| Completeness [%] | 97.2 | 98 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | sodium tartrate, PEG 3350 |
