7X45
Grass carp interferon gamma related
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 291.15 |
Detector technology | CCD |
Collection date | 2021-11-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9979 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 48.580, 48.580, 208.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.070 - 2.260 |
Rwork | 0.221 |
R-free | 0.26830 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.070 | 2.330 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.112 | 1.146 |
Number of reflections | 18555 | 16390 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 99.8 | |
Redundancy | 17.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.5 | 30% (v/v) PEG 400, 100 mM sodium cacodylate/Hydrochloric acid PH 6.5, 200 mM Lithium sulfat |