7X39

Structure of CIZ1 bound ERH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	97
Detector technology	PIXEL
Collection date	2021-06-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97856
Spacegroup name	P 1
Unit cell lengths	44.451, 48.668, 73.879
Unit cell angles	85.83, 75.08, 63.31

Refinement procedure

Resolution	38.078 - 2.850
R-factor	0.221
Rwork	0.218
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz7
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.430	3.000
High resolution limit [Å]	2.850	2.850
Number of reflections	12023
<I/σ(I)>	5.4
Completeness [%]	96.6
Redundancy	3.6
CC(1/2)	0.985	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Bis-tris propane pH 9.0, 20% v/v Jeffamine ED-2001 pH 7.0, 6% glycerol