7X39
Structure of CIZ1 bound ERH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 97 |
Detector technology | PIXEL |
Collection date | 2021-06-09 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 |
Unit cell lengths | 44.451, 48.668, 73.879 |
Unit cell angles | 85.83, 75.08, 63.31 |
Refinement procedure
Resolution | 38.078 - 2.850 |
R-factor | 0.221 |
Rwork | 0.218 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wz7 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.430 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 12023 | |
<I/σ(I)> | 5.4 | |
Completeness [%] | 96.6 | |
Redundancy | 3.6 | |
CC(1/2) | 0.985 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Bis-tris propane pH 9.0, 20% v/v Jeffamine ED-2001 pH 7.0, 6% glycerol |