7X2R

Crystal structure of the E. coli transcription termination factor Rho

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-01
Detector	AGILENT EOS CCD
Wavelength(s)	0.988
Spacegroup name	C 1 2 1
Unit cell lengths	159.496, 101.471, 204.818
Unit cell angles	90.00, 102.69, 90.00

Refinement procedure

Resolution	19.990 - 4.400
R-factor	0.2765
Rwork	0.272
R-free	0.31480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xav
RMSD bond length	0.005
RMSD bond angle	1.089
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	4.530
High resolution limit [Å]	4.400	4.400
Number of reflections	170098	7
<I/σ(I)>	0.8
Completeness [%]	86.0
Redundancy	5
CC(1/2)	0.800	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.1	0.4 M potassium chloride, 0.05 M magnesium chloride, 50 mM Tris pH 7.5, 13% (w/v) polyethylene glycol (PEG) 6000