7X2P
Legionella pneumophila effector RavL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.377, 54.247, 128.209 |
Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
Resolution | 33.150 - 2.890 |
R-factor | 0.2893 |
Rwork | 0.284 |
R-free | 0.33680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7a16 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.062 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.150 | 10.000 |
High resolution limit [Å] | 2.890 | 2.890 |
Number of reflections | 9974 | 9974 |
<I/σ(I)> | 0.1 | |
Completeness [%] | 85.0 | |
Redundancy | 10 | |
CC(1/2) | 0.840 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES sodium pH 7.5, 0.8M potassium tartate |