7X2L

Crystal structure of nanobody 3-2A2-4 with SARS-CoV-2 RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-31
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	89.168, 89.168, 129.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.330 - 2.400
R-factor	0.233
Rwork	0.230
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.330	2.488
High resolution limit [Å]	2.400	2.400
Number of reflections	20428	1695
<I/σ(I)>	23.43
Completeness [%]	97.4
Redundancy	22.1
CC(1/2)	0.991	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1M DL-Malic acid pH7.0, 12% w/v Polyethylene glycol 3350