7X2J

Crystal structure of nanobody Nb70 with SARS-CoV RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 31 2 1
Unit cell lengths	108.413, 108.413, 94.119
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.240 - 2.400
R-factor	0.1987
Rwork	0.197
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ajf
RMSD bond length	0.008
RMSD bond angle	0.945
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.240	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	25379	2496
<I/σ(I)>	25.45
Completeness [%]	99.8
Redundancy	17.9
CC(1/2)	0.996	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	291.15	0.2M Ammonium sulfate, 0.1M Bis-Tris pH 4.4, 21% w/v Polyethylene glycol 3350