7X2E
Structure of USH1C PDZ2 and coiled-coil in complex with CDHR2 C-terminal tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 54.516, 50.681, 72.847 |
Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
Resolution | 28.260 - 1.850 |
R-factor | 0.1825 |
Rwork | 0.181 |
R-free | 0.21620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2kbs |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.880 |
High resolution limit [Å] | 1.850 | 5.010 | 1.850 |
Rmerge | 0.107 | 0.040 | 0.893 |
Rmeas | 0.117 | 0.044 | 0.968 |
Rpim | 0.046 | 0.017 | 0.369 |
Total number of observations | 102320 | ||
Number of reflections | 16311 | 853 | 809 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 99.9 | 99.6 | 99.8 |
Redundancy | 6.3 | 6 | 6.5 |
CC(1/2) | 0.999 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% w/v polyethylene glycol 3000, 100 mM tri-sodium citrate, pH 5.5. |