7X1N

Crystal structure of MEF2D-MRE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-10
Detector	MAR CCD 130 mm
Wavelength(s)	0.9999
Spacegroup name	P 21 21 2
Unit cell lengths	74.863, 75.651, 111.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.992 - 3.315
R-factor	0.2439
Rwork	0.242
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6byy
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.992	3.434
High resolution limit [Å]	3.315	3.320
Rmerge	0.096	1.070
Number of reflections	9418	607
<I/σ(I)>	4.8
Completeness [%]	99.9
Redundancy	10.2
CC(1/2)	0.990	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	PEG 2000