7X0R

Crystal structure of substrate binding protein Lbp complexed wtih guanosine from Clostridium thermocellum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	53.830, 62.090, 81.300
Unit cell angles	90.00, 101.70, 90.00

Refinement procedure

Resolution	31.045 - 1.470
R-factor	0.1569
Rwork	0.157
R-free	0.17360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fqx
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.045	31.045	1.510
High resolution limit [Å]	1.470	6.570	1.470
Rmerge	0.046	0.034	0.373
Rmeas	0.062	0.048	0.508
Total number of observations	293817
Number of reflections	165622	1835	11830
<I/σ(I)>	9.73	20.61	2.15
Completeness [%]	94.4	92.9	91.3
Redundancy	1.774	1.782	1.615
CC(1/2)	0.996	0.994	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M zinc acetate, 16% w/v polyethylene glycol 3350