7X0O
Crystal structure of sugar binding protein CbpC from Clostridium thermocellum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.810, 80.060, 170.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.587 - 2.000 |
R-factor | 0.2323 |
Rwork | 0.231 |
R-free | 0.25970 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.587 | 42.587 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.087 | 0.045 | 0.688 |
Rmeas | 0.102 | 0.052 | 0.845 |
Total number of observations | 365052 | ||
Number of reflections | 103492 | 1311 | 5606 |
<I/σ(I)> | 9.5 | 19.32 | 1.47 |
Completeness [%] | 85.6 | 97.4 | 62.7 |
Redundancy | 3.527 | 3.716 | 2.482 |
CC(1/2) | 0.995 | 0.995 | 0.441 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3350 |