7X0N

Crystal structure of sugar binding protein CbpB complexed wtih laminaribiose from Clostridium thermocellum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	72.750, 46.440, 116.490
Unit cell angles	90.00, 106.58, 90.00

Refinement procedure

Resolution	25.707 - 1.680
R-factor	0.1743
Rwork	0.173
R-free	0.20990
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.707	25.707	1.720
High resolution limit [Å]	1.680	7.510	1.680
Rmerge	0.046	0.023	0.426
Rmeas	0.064	0.032	0.593
Total number of observations	278714
Number of reflections	148751	1588	11347
<I/σ(I)>	11.25	26.08	2.43
Completeness [%]	89.2	85	91.3
Redundancy	1.874	1.831	1.875
CC(1/2)	0.997	0.997	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2% Tacsimate pH 4.0, 0.1 M sodium acetate trihydrate pH 4.6, 16% w/v Polyethylene glycol 3350