7X0K
Crystal structure of sugar binding protein CbpB complexed wtih cellotriose from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.440, 44.150, 113.060 |
| Unit cell angles | 90.00, 103.98, 90.00 |
Refinement procedure
| Resolution | 40.958 - 1.700 |
| R-factor | 0.1806 |
| Rwork | 0.180 |
| R-free | 0.21560 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.958 | 40.958 | 1.740 |
| High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
| Rmerge | 0.114 | 0.073 | 0.809 |
| Rmeas | 0.133 | 0.086 | 0.950 |
| Number of reflections | 136991 | 1507 | 10092 |
| <I/σ(I)> | 6.88 | 16.96 | 1.54 |
| Completeness [%] | 95.6 | 94.4 | 95.8 |
| Redundancy | 3.485 | 3.617 | 3.497 |
| CC(1/2) | 0.993 | 0.989 | 0.603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.2 M Ammonium phosphate monobasic, pH 4.6, 22% w/v Polyethylene glycol 3350 |
