7X0F
Structure of Pseudomonas NRPS protein, AmbB-TC bound to Ppant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-31 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.411, 71.190, 172.770 |
| Unit cell angles | 90.00, 109.93, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.200 |
| Rwork | 0.210 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7x0e |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0261) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.560 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 114170 | 5650 |
| <I/σ(I)> | 11.4 | 2.1 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.4 | 2.6 |
| CC(1/2) | 0.997 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M MES pH 6.6, 0.2M ammonium nitrate, 15% PEG 3350, 5% glycerol |
