7X0D
Crystal structure of phospholipase A1, CaPLA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2013-10-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0082 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.313, 59.568, 147.319 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 49.106 - 2.397 |
| R-factor | 0.209146720375 |
| Rwork | 0.206 |
| R-free | 0.26015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yij |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.545 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.390 | 2.400 |
| Rmerge | 0.119 | 0.589 |
| Number of reflections | 67379 | 3305 |
| <I/σ(I)> | 13.97 | 2.79 |
| Completeness [%] | 96.1 | 95.5 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES (pH 7.5), 0.2M ammonium sulfate, 10% PEG 8000, 10% 2-propanol |
