7X0C
Crystal structure of phospholipase A1, AtDSEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2012-06-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0082 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.977, 95.343, 79.278 |
| Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
| Resolution | 33.287 - 1.799 |
| R-factor | 0.179912963409 |
| Rwork | 0.178 |
| R-free | 0.21173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yij |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.127 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.799 | 1.800 |
| Rmerge | 0.086 | 0.400 |
| Number of reflections | 105892 | 4586 |
| <I/σ(I)> | 35.29 | 4.7 |
| Completeness [%] | 99.8 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02M calcium chloride, 0.1M sodium acetate, 30% MPD |
