7WZM

Crystal structure of Cytochrome P450 184A1 from streptomyces avermitilis in complex with Oleic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.687, 62.652, 132.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.520 - 1.680
R-factor	0.1895
Rwork	0.188
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wzl
RMSD bond length	0.012
RMSD bond angle	1.853
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.680	1.680
Number of reflections	52789	52789
<I/σ(I)>	36.567
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.949	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288.15	15% PEG3350, 0.2 M sodium chloride, 0.1M HEPES, pH 7.0, 5% MPD