7WZE

Structure of Transcriptional regulator from Bacillus subtilis (strain 168)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	46.178, 76.545, 88.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.020 - 2.650
R-factor	0.2158
Rwork	0.211
R-free	0.29650
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.971
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Number of reflections	9627	478
<I/σ(I)>	55
Completeness [%]	99.7
Redundancy	13.4
CC(1/2)	1.000	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2 Na acetate, 0.1 Tris, 9.0, 14% glycerol, 32% PEG 4K