7WZB

lipopolysaccharide assembly protein LapB (open)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-04-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9175
Spacegroup name	P 21 2 21
Unit cell lengths	72.080, 78.960, 124.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.460 - 2.700
Rwork	0.248
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zlh
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.720	2.796
High resolution limit [Å]	2.700	2.700
Rmerge	0.042	0.231
Rmeas	0.045	0.248
Rpim	0.017	0.089
Number of reflections	19835	1537
<I/σ(I)>	27.93	7.08
Completeness [%]	97.0	78.14
Redundancy	7.3	7.08
CC(1/2)	1.000	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	293	21.25% Ethylene glycol 15% glycerol