7WXS
Lysozyme protected by polyacrylamide gel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2021-06-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.12 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.114, 79.114, 37.058 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.020 - 1.250 |
R-factor | 0.1788 |
Rwork | 0.178 |
R-free | 0.19480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lt5 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.766 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.020 | 1.700 | 1.290 |
High resolution limit [Å] | 1.250 | 1.570 | 1.250 |
Rmerge | 0.119 | 0.186 | 0.571 |
Rmeas | 0.130 | 0.203 | 0.623 |
Number of reflections | 32552 | 3245 | 3112 |
<I/σ(I)> | 23.4 | 15.5 | 3.6 |
Completeness [%] | 98.3 | 98.3 | 98.3 |
Redundancy | 6 | 6 | 6 |
CC(1/2) | 0.994 | 0.970 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 50mM sodium acetate, 1.5M NaCl, pH 4.5 |