7WXR
Crystal Structure of PL-5 family polysaccharide lyase PanPL-N171L mutant from Pandoraea apista in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2019-12-09 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.951, 52.859, 119.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.640 - 1.880 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wxj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.783 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.640 | 1.980 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.080 | 0.448 |
Number of reflections | 24617 | 3190 |
<I/σ(I)> | 27.93 | 4.55 |
Completeness [%] | 98.9 | 92.2 |
Redundancy | 14.42 | 4.28 |
CC(1/2) | 0.998 | 0.727 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 295.15 | 0.1 M Citric acid pH 3.5, 25% w/v Polyethylene glycol 3,350 |