7WXM
Crystal Structure of PL-5 family polysaccharide lyase PanPL from Pandoraea apista at pH6.5 in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07234 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.106, 84.624, 91.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.090 - 1.230 |
R-factor | 0.1705 |
Rwork | 0.170 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wxj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.063 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.090 | 1.256 |
High resolution limit [Å] | 1.230 | 1.230 |
Rmerge | 0.054 | 0.658 |
Rpim | 0.016 | 0.324 |
Number of reflections | 78113 | 2704 |
<I/σ(I)> | 21.9 | 2.1 |
Completeness [%] | 96.1 | 67.8 |
Redundancy | 11.3 | 4.9 |
CC(1/2) | 1.000 | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295.15 | 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350 |