7WXJ
Crystal Structure of PL-5 family polysaccharide lyase PanPL from Pandoraea apista at pH3.5 in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07234 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.006, 46.096, 64.586 |
Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
Resolution | 64.570 - 2.100 |
R-factor | 0.1915 |
Rwork | 0.189 |
R-free | 0.22970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7fhx |
RMSD bond length | 0.003 |
RMSD bond angle | 0.487 |
Data reduction software | XDS (Jan 26, 2018 BUILT=20180409) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.575 | 2.136 |
High resolution limit [Å] | 2.099 | 2.099 |
Rmerge | 0.076 | 0.146 |
Rpim | 0.036 | 0.099 |
Number of reflections | 15375 | 408 |
<I/σ(I)> | 16.7 | 6.4 |
Completeness [%] | 89.2 | 48.9 |
Redundancy | 5.1 | 2.9 |
CC(1/2) | 0.996 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 295.15 | 0.1 M Citric acid pH 3.5, 25% w/v Polyethylene glycol 3,350 |