7WWY
M117L variant of Cu/Zn-superoxide dismutase from dog (Canis familiaris)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.275 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 50.643, 50.643, 282.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.710 - 1.500 |
R-factor | 0.25725 |
Rwork | 0.256 |
R-free | 0.28740 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.771 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.850 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 60359 | 8347 |
<I/σ(I)> | 27 | |
Completeness [%] | 99.5 | |
Redundancy | 32.3 | |
CC(1/2) | 1.000 | 0.533 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2M Sodium malonate, pH 7.0 |