7WWW

Crystal Structure of Moonlighting GAPDH protein of Lactobacillus gasseri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	I 41 2 2
Unit cell lengths	114.922, 114.922, 118.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.673 - 1.880
Rwork	0.134
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lvf
RMSD bond length	0.007
RMSD bond angle	1.404
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.950
High resolution limit [Å]	1.880	1.880
Rmerge	0.067	0.546
Rmeas	0.070	0.572
Rpim	0.020	0.165
Number of reflections	32168	3031
<I/σ(I)>	41.7	3.7
Completeness [%]	98.8	94.8
Redundancy	12	10.5
CC(1/2)		0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M sodium cacodylate pH 6.5, 16% (w/v) PEG 4000