7WWR
Structure of a triple-helix region of human collagen type III from Trautec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.809, 21.325, 57.636 |
| Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
| Resolution | 23.772 - 1.300 |
| Rwork | 0.137 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cuo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.034 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.760 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.037 | 0.067 |
| Number of reflections | 14504 | 738 |
| <I/σ(I)> | 35.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MIB pH5.0, 25% PEG 1500 |
