7WWO
Dimer form of hypothetical protein TTHA1873 from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0723 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 90.422, 90.422, 141.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 68.630 - 2.170 |
R-factor | 0.1874 |
Rwork | 0.186 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wrk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.590 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.630 | 2.240 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 18800 | 1575 |
<I/σ(I)> | 5.6 | |
Completeness [%] | 99.9 | |
Redundancy | 9.9 | |
CC(1/2) | 0.959 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 60% v/v Tacsimate pH 7.0 |