7WWD
Crystal structure of Saccharomyces cerevisiae Sfh2 complexed with squalene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2021-07-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.403, 78.278, 54.166 |
Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
Resolution | 32.660 - 2.390 |
R-factor | 0.2059 |
Rwork | 0.200 |
R-free | 0.26220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.719 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.101 | 0.416 |
Rpim | 0.050 | |
Number of reflections | 13881 | 700 |
<I/σ(I)> | 25.1 | 5.8 |
Completeness [%] | 97.3 | 100 |
Redundancy | 4 | 4.1 |
CC(1/2) | 0.992 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES pH7.0 0.1M KNO3, 30% PEG 3350 |