7WWD

Crystal structure of Saccharomyces cerevisiae Sfh2 complexed with squalene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	110
Detector technology	CCD
Collection date	2021-07-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	43.403, 78.278, 54.166
Unit cell angles	90.00, 94.11, 90.00

Refinement procedure

Resolution	32.660 - 2.390
R-factor	0.2059
Rwork	0.200
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.719
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.390	2.390
Rmerge	0.101	0.416
Rpim	0.050
Number of reflections	13881	700
<I/σ(I)>	25.1	5.8
Completeness [%]	97.3	100
Redundancy	4	4.1
CC(1/2)	0.992	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES pH7.0 0.1M KNO3, 30% PEG 3350