7WWC

Crystal structure of beta-1,3(4)-glucanase with Laminaritriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-17
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	120.648, 120.648, 69.966
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.860 - 2.202
R-factor	0.194771287542
Rwork	0.191
R-free	0.24224
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7mm4
RMSD bond length	0.009
RMSD bond angle	0.996
Data scaling software	HKL-3000
Refinement software	PHENIX (dev_2474)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.867	2.280
High resolution limit [Å]	2.200	2.200
Rmeas	0.153
Number of reflections	29797	2786
<I/σ(I)>	1.5
Completeness [%]	95.0
Redundancy	18.9
CC(1/2)		0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tris pH8.5, 1.2M Potassium sodium tartrate