7WW9
Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 1.5 hr in 20 mM Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.962, 55.906, 59.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.940 - 1.800 |
| R-factor | 0.1616 |
| Rwork | 0.161 |
| R-free | 0.17800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3a6t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.940 | 1.970 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.072 | 0.336 |
| Number of reflections | 12148 | 1171 |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |
