7WW8
Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 5 hr in 5 mM Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.264, 56.051, 59.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.690 - 1.640 |
| R-factor | 0.1572 |
| Rwork | 0.156 |
| R-free | 0.18120 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3a6t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.258 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.690 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.062 | 0.220 |
| Number of reflections | 16066 | 1534 |
| <I/σ(I)> | 41.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |
