7WW7
Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 1 hr in 5 mM Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-07 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.278, 55.968, 59.181 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.600 - 1.670 |
R-factor | 0.1612 |
Rwork | 0.159 |
R-free | 0.19800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3a6t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.238 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.600 | 1.730 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.089 | 0.303 |
Number of reflections | 15328 | 1483 |
<I/σ(I)> | 29.3 | |
Completeness [%] | 99.6 | |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |