7WW3

Crystal structure of MmIMP1-KH34 tandem domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	P 61 2 2
Unit cell lengths	59.315, 59.315, 189.318
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.840 - 1.900
R-factor	0.2413
Rwork	0.239
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3krm
RMSD bond length	0.009
RMSD bond angle	1.062
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.075	1.471
Rmeas	0.076	1.490
Rpim	0.012	0.237
Number of reflections	16426	2311
<I/σ(I)>	30.2	3
Completeness [%]	99.8	99.6
Redundancy	37	37.6
CC(1/2)	1.000	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4000