7WUX
Crystal structure of AziU3/U2 complexed with (5S,6S)-O7-sulfo DADH from Streptomyces sahachiroi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2021-10-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.872, 131.756, 157.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.873 - 1.800 |
Rwork | 0.164 |
R-free | 0.19340 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 1.196 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.873 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.260 |
Rmeas | 0.060 | 0.283 |
Rpim | 0.032 | 0.110 |
Number of reflections | 106322 | 5231 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 99.6 | |
Redundancy | 6.3 | 6.5 |
CC(1/2) | 0.999 | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 20% PEG 3350, 0.2M Magnesium formic acid |