7WUL
Crystal structure of UBR bof from PRT6 (RDG pH5.5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-07-25 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 29.802, 29.802, 114.856 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.180 - 1.580 |
R-factor | 0.1628 |
Rwork | 0.160 |
R-free | 0.19280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1300027541 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.640 |
High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
Rmerge | 0.058 | 0.047 | 0.121 |
Rmeas | 0.063 | 0.052 | 0.130 |
Rpim | 0.024 | 0.021 | 0.046 |
Total number of observations | 65939 | ||
Number of reflections | 8723 | 961 | 839 |
<I/σ(I)> | 10.3 | ||
Completeness [%] | 99.5 | 96.9 | 99.5 |
Redundancy | 7.6 | 6.3 | 8.2 |
CC(1/2) | 0.997 | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M sodium citrate, 200mM NaCl, 0.1M Tris |