7WT6
Crystal structure of full-length peptidyl-tRNA hydrolase from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976254 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.581, 39.003, 123.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.200 - 1.940 |
R-factor | 0.1712 |
Rwork | 0.169 |
R-free | 0.21450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z2i |
RMSD bond length | 0.006 |
RMSD bond angle | 0.793 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.201 | 1.970 |
High resolution limit [Å] | 1.937 | 1.937 |
Rmerge | 0.149 | 0.720 |
Rmeas | 0.159 | 0.780 |
Rpim | 0.055 | 0.290 |
Number of reflections | 13646 | 587 |
<I/σ(I)> | 11.4 | 2.5 |
Completeness [%] | 98.6 | 87.7 |
Redundancy | 8 | 6.3 |
CC(1/2) | 0.996 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293.15 | 25% (W/V) PEG 8000, 0.1M HEPES pH 7.5 5% (V/V) 2-propanol |