7WT2
human glyoxalase I in complex with TLSC702
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.691, 82.532, 45.281 |
| Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.000 |
| R-factor | 0.1873 |
| Rwork | 0.183 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vw9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 | 0.262 |
| Number of reflections | 25974 | 3727 |
| <I/σ(I)> | 13.9 | 4 |
| Completeness [%] | 96.9 | 97 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.999 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M NaCl, 0.1 M Bis-Tris, 25% PEG3350 |
