7WRS

Crystal structure of the chicken isoleucyl-tRNA synthetase 1 (IARS1) UNE-I complexed with glutamyl-tRNA synthetase 1 (EARS1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	PAL-XFEL BEAMLINE NCI
Synchrotron site	PAL-XFEL
Beamline	NCI
Temperature [K]	293
Detector technology	CCD
Collection date	2019-03-22
Detector	RAYONIX MX225-HS
Wavelength(s)	1.2782
Spacegroup name	P 21 21 2
Unit cell lengths	143.020, 155.550, 44.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.510 - 2.400
R-factor	0.194
Rwork	0.191
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3z
RMSD bond length	0.009
RMSD bond angle	0.978
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.14-3260_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.800	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	39660	3908
<I/σ(I)>	5.2
Completeness [%]	100.0
Redundancy	622.9
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	0.84 M NaH2PO4, 1.76 M K2HPO4, and 0.1 M sodium acetate, pH 4.5