7WRK
Structure of hypothetical protein TTHA1873 from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-06-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.180, 42.180, 156.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.770 - 1.780 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.19850 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.827 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.300 | 1.810 |
High resolution limit [Å] | 1.780 | 1.780 |
Number of reflections | 14566 | 804 |
<I/σ(I)> | 12.6 | |
Completeness [%] | 100.0 | |
Redundancy | 11.8 | |
CC(1/2) | 0.995 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | 0.1 M Tris pH 8.5 and 3.0 M Sodium chloride |