7WQY

Crystal structure of grass carp ARF1-GDP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	121.571, 43.688, 87.841
Unit cell angles	90.00, 131.53, 90.00

Refinement procedure

Resolution	18.110 - 1.950
R-factor	0.1903
Rwork	0.186
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bh7
Data reduction software	autoPROC
Data scaling software	xia2
Phasing software	SHELXS
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.110	2.023
High resolution limit [Å]	1.950	1.950
Number of reflections	24332	2167
<I/σ(I)>	12.65
Completeness [%]	95.8
Redundancy	2
CC(1/2)	0.997	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289.15	0.1M Sodium citrate tribasic dihydrate, pH 5.5, 22% polyethylene glycol 1000