7WOL
Crystal structure of lipase TrLipB from Thermomocrobium roseum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 129.402, 129.402, 122.792 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.750 - 2.000 |
Rwork | 0.157 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lzk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.454 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.750 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.821 |
Number of reflections | 70819 | 4504 |
<I/σ(I)> | 22.8 | |
Completeness [%] | 100.0 | |
Redundancy | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1M Succinic acid,0.1M HEPES,7,1% w/vPEG 2000 MME |