7WNT

RNase J from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-06-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97930
Spacegroup name	P 64 2 2
Unit cell lengths	128.520, 128.520, 184.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.640 - 2.440
R-factor	0.2208
Rwork	0.219
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zq4
RMSD bond length	0.009
RMSD bond angle	1.026
Data reduction software	XDS (1.13_2998)
Data scaling software	XDS (1.13_2998)
Phasing software	PHENIX (1.10-2155-000)
Refinement software	PHENIX (1.10-2155-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	2.520
High resolution limit [Å]	2.440	2.440
Rmerge	0.016	0.259
Number of reflections	33845	3269
<I/σ(I)>	22.93	2.43
Completeness [%]	99.7
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M HEPES (pH 7.5) 4.3 M NaCl