7WNJ
Crystal structure and Cap Binding analysis of the methyltransferase of Langat virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.978910 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 35.722, 91.554, 98.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.780 - 1.700 |
R-factor | 0.1817 |
Rwork | 0.181 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oxt |
RMSD bond length | 0.006 |
RMSD bond angle | 0.737 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.780 | 1.763 |
High resolution limit [Å] | 1.700 | 1.702 |
Number of reflections | 35880 | 3143 |
<I/σ(I)> | 25.89 | |
Completeness [%] | 98.8 | |
Redundancy | 12.31 | |
CC(1/2) | 0.990 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% glycerol; 0.1 M Tris, pH 8.0; and 20% PEG 3350 |