7WNB
Crystal structure of SARS-CoV-2 spike receptor-binding domain (RBD) in complex with NCV2SG48 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 87.297, 102.943, 114.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.648 - 2.180 |
| R-factor | 0.2467 |
| Rwork | 0.245 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eam 7chp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.940 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 54347 | 4404 |
| <I/σ(I)> | 5.1 | 1.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.1 | |
| CC(1/2) | 0.948 | 0.316 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5 M ammonium sulfate, 19% PEG3350, 0.1 M Bis-Tris pH 5.5 |
