7WMK
PQQ-dependent alcohol dehydrogenase complexed with PQQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.973, 91.190, 57.332 |
Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
Resolution | 44.151 - 1.470 |
R-factor | 0.1385 |
Rwork | 0.138 |
R-free | 0.14010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wmd |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.250 | 1.540 |
High resolution limit [Å] | 1.470 | 1.470 |
Number of reflections | 95115 | 15196 |
<I/σ(I)> | 14.16 | |
Completeness [%] | 98.7 | |
Redundancy | 6.6 | |
CC(1/2) | 0.998 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 250mM Sodium acetate, 100mM BIS-Tris (pH 7.5), 150mM NaCl, 15% PEG 3350 |